The Coastal Atmosphere and Sea Time Series (CoASTS) Project, aimed at supporting ocean color research and applications, from 1995 up to the time of publication of this document, has ensured the collection of a comprehensive atmospheric and marine data set from an oceanographic tower located in the northern Adriatic Sea. The instruments and the measurement methodologies used to gather quantities relevant for bio-optical modeling and for the calibration and validation of ocean color sensors, are described. Particular emphasis is placed on four items: 1) the evaluation of perturbation effects in radiometric data (i.e., tower-shading, instrument self-shading, and bottom effects); 2) the intercomparison of seawater absorption coefficients from in situ measurements and from laboratory spectrometric analysis on discrete samples; 3) the intercomparison of two filter techniques for in vivo measurement of particulate absorption coefficients; and 4) the analysis of repeatability and reproducibility of the most relevant laboratory measurements carried out on seawater samples (i.e., particulate and yellow substance absorption coefficients, and pigment and total suspended matter concentrations). Sample data are also presented and discussed to illustrate the typical features characterizing the CoASTS measurement site in view of supporting the suitability of the CoASTS data set for bio-optical modeling and ocean color calibration and validation.